iQue® Human T Cell Killing Kit

Tech Support Documents
iQue® Human T Cell Killing Kit
 

The Human T Cell Mediated Killing Kit was designed for ease of use in multiplexing cell phenotype and function markers along with bead-based, secreted protein profile measurements in the same assay. This one wash assay requires minimal hands on time to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
  • Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD8+ T cytotoxic cells, and CD3+ CD8- T helper cells.
  • The panel also includes 2 different T cell functional markers, CD25 and PD-1.
  • Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.

Pack Size
Article number: 
97060
*Custom/bulk order quotes are provided within 72 hours of request.
iQue® Human T Cell Killing Kit
iQue® Human T Cell Killing Kit

The Human T Cell Mediated Killing Kit was designed for ease of use in multiplexing cell phenotype and function markers along with bead-based, secreted protein profile measurements in the same assay. This one wash assay requires minimal hands on time to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye.
  • Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD8+ T cytotoxic cells, and CD3+ CD8- T helper cells.
  • The panel also includes 2 different T cell functional markers, CD25 and PD-1.
  • Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.

Item No.:
97060

Overview

Specifications

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Key Benefits

  • Speed: Sample to actionable data by allowing multiple assays to be run in one assay thereby reducing the hands on time, numbers of overall tests and conserving precious sample.
  • Results: 8 endpoints from a single well assay measuring markers of T cell immunophenotypes and function, cell health, cell count, secreted pro-inflammatory cytokine IFNγ, and pro-apoptotic serine protease Granzyme B.
  • Biological insight: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.
  • Simplicity: Single platform assay and data acquisition eliminates the time of acquiring and correlating data from multiple sources.

Applications

  • Application
    Immune Cell Function
  • Research Area
    • Immuno-Oncology
    • Antibody Discovery
    • Drug Discovery

Biological Information

  • Target
    Human

General Specifications

  • Container Temperature Requirement
    2-8 °C
  • Laser Configuration
    Violet-Blue-Red
  • Reagents and Kits Type
    T Cell Mediated Killing
  • Temperature Range
    2-8 °C

Product Information

  • Brand
    iQue®
  • Pack Size
    1 X 96 wells
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